Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 5;12(1):133. doi: 10.3390/antiox12010133

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Febuxostat facilitates nuclear translocation of Nrf2. (A,C) 3T3-L1 and C3H10T1/2 cells were cultured with Omav or Febu for the indicated time period. Nrf2 levels in the nucleus were analyzed by western blotting. p84 served as a loading control. Relative changes in the band intensities normalized by respective loading controls are indicated. (B,D) mRNA expression of Ho-1 and Nqo1, the target genes of Nrf2, was determined using real-time PCR. Gapdh served as an endogenous control to normalize each sample. Data are expressed as the mean ± SD (n = 3). * p < 0.05. (E) 3T3-L1 and C3H10T1/2 cells were cultured with allopurinol (Allo) for the indicated time period. Nrf2 levels in the nucleus were analyzed by western blotting, and p84 served as a loading control. Relative changes in the band intensities normalized by respective loading controls are indicated.

Febuxostat facilitates nuclear translocation of Nrf2. (A,C) 3T3-L1 and C3H10T1/2 cells were cultured with Omav or Febu for the indicated time period. Nrf2 levels in the nucleus were analyzed by western blotting. p84 served as a loading control. Relative changes in the band intensities normalized by respective loading controls are indicated. (B,D) mRNA expression of Ho-1 and Nqo1, the target genes of Nrf2, was determined using real-time PCR. Gapdh served as an endogenous control to normalize each sample. Data are expressed as the mean ± SD (n = 3). * p < 0.05. (E) 3T3-L1 and C3H10T1/2 cells were cultured with allopurinol (Allo) for the indicated time period. Nrf2 levels in the nucleus were analyzed by western blotting, and p84 served as a loading control. Relative changes in the band intensities normalized by respective loading controls are indicated.