Figure 1.

Hyd protects LECs from H₂O₂-induced oxidative cell death. (A,B) Viability assay showing the dose-dependent effect(s) of Hyd on survival of SRA-hLECs and mLECs. Cultured SRA-hLECs and mLECs were treated with different concentrations of Hyd to determine its nontoxic concentration using MTS assay. The data represent the mean ± S.D. of three independent experiments. Untreated vs. Hyd-treated LECs; * p < 0.05; ** p < 0.001. (C,D) The cell viability of LECs under H₂O₂-induced oxidative stress was significantly improved with Hyd treatment. Cultured SRA-hLECs and mLECs were exposed to H₂O₂ in absence or presence of Hyd for 24 h as indicated in Figure 1, and cell viability was performed using MTS assay. Histograms represent the mean ± S.D. value of three independent experiments. * p < 0.05; ** p < 0.001. (E,F) Hyd defends LECs against H₂O₂-induced cell death by reducing the ROS levels. Hyd-treated and untreated SRA-hLECs and mLECs were exposed to H₂O₂. Then, 16 h later, ROS levels were quantified with H₂-DCF-DA dye method. Histogram represents the mean ± S.D. of three independent experiments. * p < 0.05; ** p < 0.001.