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. 2023 Jan 6;12(1):140. doi: 10.3390/antiox12010140

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Hyd efficiently activated the Nrf2 antioxidant signaling pathway in LECs. (A,B) Hyd increased the cellular abundance of Nrf2 and Prdx6 protein in a dose-dependent fashion, as demonstrated by Western analysis of total cell lysate. Total cell lysate was isolated from SRA-hLECs (A) and mLECs (B) treated with different concentrations of Hyd for 24 h. Nrf2 and Prdx6 protein expression were analyzed using their corresponding specific antibodies; tubulin served as internal control. Nrf2 and Prdx6 protein band were scanned and quantified using a densitometry and were normalized with corresponding tubulin levels. Histograms represent the relative density values that were depicted on the right side of the protein band. The data represent the mean ± S.D. values of three independent experiments. Untreated vs. Hyd-treated LECs; ** p < 0.001. (C–H) Hyd significantly augmented levels of Nrf2 and its major target antioxidant genes examined, such as Prdx6, NQO1, HO1, GCLC, and GCLM. LECs were treated with different concentrations of Hyd for 24 h. Total RNA were isolated and processed for real-time PCR using specific primers. Data represent mean ± S.D. values of three independent experiments. Untreated vs. Hyd-treated LECs; * p < 0.05; ** p < 0.001.

Hyd efficiently activated the Nrf2 antioxidant signaling pathway in LECs. (A,B) Hyd increased the cellular abundance of Nrf2 and Prdx6 protein in a dose-dependent fashion, as demonstrated by Western analysis of total cell lysate. Total cell lysate was isolated from SRA-hLECs (A) and mLECs (B) treated with different concentrations of Hyd for 24 h. Nrf2 and Prdx6 protein expression were analyzed using their corresponding specific antibodies; tubulin served as internal control. Nrf2 and Prdx6 protein band were scanned and quantified using a densitometry and were normalized with corresponding tubulin levels. Histograms represent the relative density values that were depicted on the right side of the protein band. The data represent the mean ± S.D. values of three independent experiments. Untreated vs. Hyd-treated LECs; ** p < 0.001. (C–H) Hyd significantly augmented levels of Nrf2 and its major target antioxidant genes examined, such as Prdx6, NQO1, HO1, GCLC, and GCLM. LECs were treated with different concentrations of Hyd for 24 h. Total RNA were isolated and processed for real-time PCR using specific primers. Data represent mean ± S.D. values of three independent experiments. Untreated vs. Hyd-treated LECs; * p < 0.05; ** p < 0.001.