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. 2023 Jan 12;12(1):183. doi: 10.3390/antiox12010183

Figure 6.

Figure 6

Functionality Analysis of CM and different fractions (SN or sEV) of the conditioned medium of F-OVCAs on OVCAs. (A) Representative images of colony formation in OVCAc treated with CM, SN or sEV from F-OVCAc for 6 days in OVCA1; (B) Histogram of cell viability by MTT assay in OVCAc treated with SN or sEV from F-OVCAc for 6 days in OVCA1; (C) Histogram of Malondialdehyde (MDA) levels in OVCAc treated with CM, SN or sEV from F-OVCAc for 6 days in OVCA1; (D) Histogram of ratio GSH/GSSG (Reduced Glutathione/Oxidized Glutathione) levels in OVCAc treated with CM, SN or sEV from F-OVCAc for 6 days in OVCA1; (E) Histogram of Iron (II) (Fe2+) levels in OVCAc treated with CM, SN or sEV from F-OVCAc for 6 days in OVCA1; (F) Histogram of reactive oxygen species (ROS) levels in OVCAc treated with CM, SN or sEV from F-OVCAc for 6 days in OVCA1; (G) Histogram of colony formation in OVCAc treated with CM, SN or sEV from F-OVCAc for 3 days in OVCA1; (H) Histogram of cell viability by MTT assay in OVCAc treated with CM, SN or sEV from F-OVCAc for 3 days in OVCA1; (I) Histogram of Malondialdehyde (MDA) levels in OVCAc treated with CM, SN or sEV from F-OVCAc for 3 days in OVCA1; (J) Histogram of ratio GSH/GSSG (Reduced Glutathione/Oxidized Glutathione) levels in OVCAc treated with CM, SN or sEV from F-OVCAc for 3 days in OVCA1; (K) Histogram of Iron (II) (Fe2+) levels in OVCAc treated with CM, SN or sEV from F-OVCAc for 3 days in OVCA1; (L) Histogram of reactive oxygen species (ROS) levels in OVCAc treated with CM, SN or sEV from F-OVCAc for 3 days in OVCA1. The graphs show the mean ± SD of three independent experiments. * p < 0.05 and ** p < 0.01.