Figure 8.

Determination inhibition of ferroptosis paracrine transmission using defunctionalized sEV by Triton-X. (A) Histogram of colony formation in OVCAc treated with sEV from F-OVCAc for 6 days using crystal violet assay; (B) Histogram of cell viability using MTT assay in OVCAc treated with sEV with/without pre-treatment with Triton-X from F-OVCAc for 6 days in OVCA1; (C) Histogram of Malondialdehyde (MDA) levels in OVCAc treated with sEV with/without pre-treatment with Triton-X from F-OVCAc for 6 days in OVCA1; (D) Histogram of Iron (II) (Fe²⁺) levels in OVCAc treated with sEV with/without pre-treatment with Triton-X from F-OVCAc for 6 days in OVCA1; (E) Histogram of reactive oxygen species (ROS) levels in OVCAc treated sEV with/without pre-treatment with Triton-X from F-OVCAc for 6 days in OVCA1. The graphs show the mean ± SD of three independent experiments. ** p < 0.01.