Figure 10.
Determination inhibition for ferroptosis paracrine transmission by sEV in OVCAc. (A) Histogram of colony formation in OVCAc treated with sEV from F-OVCAc for 6 days using crystal violet assay; (B) Histogram of cell viability using MTT assay in OVCAc treated with sEV from F-OVCAc with/without ferroptotic inhibitors (Ferrostatin-1 (Fer-1) and Deferoxamine (DFO)) for 6 days in OVCA1; (C) Histogram of Malondialdehyde (MDA) levels in OVCAc treated with sEV from F-OVCAc with/without ferroptotic inhibitors (Fer-1 and DFO) for 6 days in OVCA1; (D) Histogram of Iron (II) (Fe2+) levels in OVCAc treated with sEV from F-OVCAc with/without ferroptotic inhibitors (Fer-1 and DFO) for 6 days in OVCA1; (E) Histogram of reactive oxygen species (ROS) levels in OVCAc treated with sEV from F-OVCAc with/without ferroptotic inhibitors (Fer-1 and DFO) for 6 days in OVCA1. The graphs show the mean ± SD of three independent experiments. * p < 0.05 and ** p < 0.01.