Figure 12.

Determination inhibition after the ferroptosis paracrine transmission by sEV in OVCAc. (A) Histogram of colony formation using crystal violet assay and (B) Histogram of cell viability using MTT assay in OVCAc treated with sEV from F-OVCAc for 6 days and with/without ferroptotic inhibitors (Ferrostatin-1 (Fer-1) and Deferoxamine (DFO)) for 6 days in OVCA1; (C) Histogram of Malondialdehyde (MDA) levels in OVCAc treated with sEV from F-OVCAc for 6 days and with/without ferroptotic inhibitors (Fer-1 and DFO) for 6 days in OVCA1; (D) Histogram of Iron (II) (Fe²⁺) levels in OVCAc treated with sEV from F-OVCAc for 6 days and with/without ferroptotic inhibitors (Fer-1 and DFO) for 6 days in OVCA1; (E) Histogram of reactive oxygen species (ROS) levels in OVCAc treated with sEV from F-OVCAc for 6 days and with/without ferroptotic inhibitors (Fer-1 and DFO) for 6 days in OVCA1. The graphs show the mean ± SD of three independent experiments. ** p < 0.01.