Figure 2.

Anti-wrinkle effects of YJP-EA, Hex, and BuOH on UVB-irradiated human dermal fibroblast cells. (A) HDF cells were treated with YJP-EA, Hex, and BuOH for 24 h. An MTT assay was performed to evaluate cell viability. (B) HDF cells were irradiated with UVB (100 mJ/cm²) and then treated with YJP-EA, Hex, and BuOH for 24 h. Whole cell lysates were analyzed by Western blot analysis. (C) HDF cells were treated by YJP-EA, Hex, and BuOH for 24 h after UVB (100 mJ/cm²) irradiation. The RNA level was evaluated using reverse transcription PCR. (D) The Pro-Collagen of HDF cells was measured using an ELISA kit, following the manufacturer’s instructions, with 450 nm. All experiments were performed individually in triplicate. *** p < 0.001 vs. non-treated (NT) cells, ** p < 0.01 vs. non-treated (NT) cells, and * p < 0.05 vs. non-treated (NT) cells.