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. 2022 Dec 26;12(1):51. doi: 10.3390/antiox12010051

Figure 5.

Figure 5

Antioxidant effects of YJP-EA, -Hex, and -BuOH on HaCaT, HDF, and B16F10 cells. HaCaT and HDF cells were irradiated with UVB; B16F10 cells were stimulated by α-MSH. We then treated them with NAC (3 mM) for 15 min or with YJP-EA, -Hex, and -BuOH (50 μg/mL) for 12 h. (A–C) The ROS production was analyzed by flow cytometer. (D–F) The GSH and GSSG levels were measured, and the GSSG/GSH ratio was evaluated in both cells. All experiments were performed individually in triplicate. ### p < 0.001 vs. non-treated (NT) cells, ## p < 0.01 vs. non-treated (NT) cells, *** p < 0.001 vs. UVB or α-MSH-stimulated cells, ** p < 0.01 vs. UVB or α-MSH-stimulated cells, and * p < 0.05 vs. UVB or α-MSH-stimulated cells.