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. 2022 Dec 30;12(1):90. doi: 10.3390/antiox12010090

Figure 6.

Figure 6

PAC modulates hepatic lipid metabolism. Protein expression of pivotal biomarkers influencing lipid metabolism and biogenesis was determined in liver by Western blot as described in Materials and Methods. (A) ACC, (B) p-ACC, (D) FAS, (E) SREBP1c, (F) CPT1A, (G) PPARα, (H) PGC1α, (I) AMPKα and (J) p-AMPKα protein mass was determined. (A) ACC and (B) p-ACC as well as (I) AMPKα and (J) p-AMPKα were blotted on separate gels and normalized using their respective β-actins; the (C) p-ACC/ACC and (K) p-AMPKα/AMPKα ratios were then calculated. Data are expressed as the mean ± SEM for n = 4–8 mice/group with a representative gel per experiment. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HFHS mice. Each set of experiments, including the proteins of interest and their β-actin (as a reference protein and a loading housekeeping control) was run on the same gel. ACC: acetyl-CoA carboxylase; p-ACC: Phosphorylated-acetyl-CoA carboxylase; FAS: Fatty acid synthase; SREBP1c: Sterol regulatory element binding protein-1c; CPT1A: Carnitine palmitoyl transferase 1 isoform A; PPARα: Peroxisome proliferator activated receptor alpha; PGC1α: Peroxisome proliferator activated receptor gamma coactivator 1 alpha; AMPKα: AMP-activated protein kinaseα; p-AMPKα: Phosphorylated-AMP-activated protein kinaseα.