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. 2022 Dec 30;12(1):90. doi: 10.3390/antiox12010090

Figure 7.

Figure 7

Effect of PACs on inflammatory and OxS processes in liver HFHS-fed mice. The protein mass of (A) COX-2, (B) TNFα, (C) NF-κB and (D) IκB was determined by Western blot as described in Materials and Methods. Both (C) NF-κB and (D) IκB were blotted on the same gel and normalized using the same GAPDH; the (E) NF-kB/IκB ratio was then calculated. Moreover, the protein expression of the antioxidant defense biomarkers, namely (F) GPx, (G) SOD2 and the transcription factor nuclear factor (H) NRF2 were evaluated by Western blot as described in Materials and Methods section. Results are presented as mean ± SEM for n = 4–8 mice/group with a representative gel per experiment. * p < 0.05, ** p < 0.01 vs. HFHS mice. Each set of experiments, including the proteins of interest and their β-actin or GAPDH (as a reference protein and a loading housekeeping control) was run on the same gel.