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. 2023 Jan 10;12(1):163. doi: 10.3390/antiox12010163

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Photographic records of whole-cell lyophilizates after three months of storage at 4 °C (A1), after three months of storage at 37 °C and 90% relative humidity (A2), after three months of storage at −80 °C and 48 h at ambient temperature (A3), and after γ-irradiation at 50 kGy (A4). Complex viscosity η* value evolution at various timepoints after non-irradiated (B) and γ-irradiated (31 kGy, (C)) whole-cell lyophilizate reconstitution in HA-based hydrogels (2.2–2.4 MDa MW) within an oxidative challenge assay. Extremely significant (i.e., **** or p value < 0.0001) differences were found between each challenged sample and the corresponding unchallenged control. HA, hyaluronic acid; kGy, kiloGray; LYO-WC, lyophilized whole-cell fraction; MDa, megadalton; MW, molecular weight; Pa·s, Pascal seconds.