Table 2.
Loss factor tan δ 1 values of lyophilized whole-cell samples resuspended in various HA-based hydrogels and challenged with various doses of H2O2 for 1 h, corresponding to the complex viscosity η* values presented in Figure 5A–C. The mean values are presented for each group and assay condition along with the corresponding standard deviations. BSA, bovine serum albumin; HA, hyaluronic acid; MDa, megaDalton; MW, molecular weight; PBS, phosphate-buffered saline.
| Challenge Item Concentration (H2O2% w/w) | ||||||||
|---|---|---|---|---|---|---|---|---|
| 0% | 5% | 10% | 15% | 20% | 25% | 30% | ||
| HA 1.0–1.25 MDa MW | PBS Test Item | 5.714 ± 0.176 | 6.728 ± 0.254 | 7.854 ± 0.450 | 9.509 ± 0.568 | 10.284 ± 1.378 | 11.343 ± 1.971 | 15.522 ± 3.839 |
| Whole-Cell Test Item | 3.468 ± 0.612 | 1.396 ± 0.119 | 1.427 ± 0.382 | 1.448 ± 0.188 | 1.435 ± 0.090 | 1.552 ± 0.155 | 1.677 ± 0.109 | |
| HA 2.2–2.4 MDa MW | PBS Test Item | 1.189 ± 0.054 | 1.309 ± 0.084 | 1.478 ± 0.133 | 1.559 ± 0.095 | 1.651 ± 0.098 | 1.733 ± 0.127 | 1.623 ± 0.414 |
| Whole-Cell Test Item | 1.059 ± 0.176 | 0.735 ± 0.068 | 0.704 ± 0.182 | 0.689 ± 0.184 | 0.680 ± 0.160 | 0.690 ± 0.222 | 0.761 ± 0.049 | |
| BSA Test Item | 0.995 ± 0.117 | 1.056 ± 0.121 | 1.050 ± 0.062 | 1.072 ± 0.088 | 1.101 ± 0.085 | 1.124 ± 0.136 | 1.128 ± 0.097 | |
1 Tan δ values were obtained by dividing the measured loss modulus G″ of the system by the storage modulus G′ of the system.