Table 3.
Proteomic analysis of samples at different steps of the manufacturing process and following gamma irradiation at various doses. Detected protein concentrations are presented in pg/mL of samples in decreasing relative order of quantitative detection in the fresh cell lysate group. The mean values are presented for each group and each assay condition, along with the corresponding standard deviations. kGy, kiloGray; LYO-LYS, lyophilized cell lysate fraction; LYS, lysate fraction.
| Protein Name | Sample Groups | ||||
|---|---|---|---|---|---|
| LYS (pg/mL) | LYO-LYS (%) 1 | LYO-LYS; 5 kGy (%) 1 | LYO-LYS; 25 kGy (%) 1 | LYO-LYS; 50 kGy (%) 1 | |
| MMP2 | 11,296 ± 2,620 | 23 ± 29 | 1 ± 0 | −26 ± 19 | −32 ± 1 |
| sEGFR | 8,300 ± 803 | 29 ± 7 | 33 ± 7 | 114 ± 31 | 58 ± 5 |
| TIMP1 | 3,419 ± 575 | 15 ± 27 | 20 ± 15 | 69 ± 26 | 45 ± 37 |
| MCSF | 2,737 ± 182 | 10 ± 3 | −11 ± 6 | −12 ± 9 | −22 ± 5 |
| Follistatin | 445 ± 25 | −54 ± 23 | −13 ± 3 | 185 ± 7 | 221 ± 3 |
| Endoglin | 431 ± 41 | 8 ± 23 | 50 ± 8 | 43 ± 2 | 37 ± 13 |
| IL-1Ra | 364 ± 46 | 7 ± 3 | −7 ± 2 | −36 ± 4 | −48 ± 6 |
| IL-16 | 177 ± 35 | −25 ± 0 | 8 ± 5 | 169 ± 28 | 107 ± 4 |
| FGF-1 | 191 ± 35 | −10 ± 18 | 223 ± 8 | 1,312 ± 23 | 1,423 ± 6 |
| IL-23 | 115 ± 77 | 40 ± 29 | −18 ± 20 | −41 ± 9 | −55 ± 37 |
| sIL-6R | 112 ± 8 | 13 ± 5 | −2 ± 1 | −16 ± 19 | −33 ± 0 |
| SDF1a-b | 52 ± 14 | −18 ± 49 | 47 ± 13 | 199 ± 12 | 340 ± 6 |
| VEGF-C | 29 ± 3 | −14 ± 9 | 121 ± 10 | 343 ± 6 | 333 ± 13 |
| TPO | 13 ± 6 | 7 ± 40 | 88 ± 7 | 3,834 ± 63 | 1,794 ± 8 |
1 The data for the lyophilized cell lysate (i.e., LYO-LYS) and γ-irradiated lyophilized cell lysate (i.e., LYO-LYS; 5–50 kGy) fractions are presented as net absolute percentages of change in the detected protein levels compared to the quantitative data presented for the non-lyophilized and non-irradiated cell lysate fraction (i.e., LYS).