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. 2022 Dec 26;12(1):42. doi: 10.3390/antiox12010042

Figure 4.

Figure 4

HoloBet v 1 decreased antigen presentation is associated with an increase of labile iron in monocytic cells. Flowcytometric analyses of PBMCs (n = 34) stimulated with medium, iron-quercetin (FeQ2), apoBet v 1 alone or in combination thereof (holoBet v 1). (a): gating strategy and relative numbers of CD14+HLADR+CD86+ in the monocytic gate and (b): the respected MFI of the calcein signal. Linear regression analysis of the Calcein signal of CD14+ cells (c): versus CD86 expression and (d): HLADR expression as well as (e): calcein-signal of CD14+ with the relative numbers of CD14+HLADR+CD86+ cells. Data were analysed in (a,b) by mixed-effect analysis. All data were Geisser-Greenhouse corrected and followed by Tukey’s multiple comparisons test. (ce). Simple linear regression models of data with 95% confidence bands are depicted. * p < 0.05; *** p < 0.001; **** p < 0.0001; ns = not significant.