Skip to main content
. 2022 Dec 22;12(1):15. doi: 10.3390/antibiotics12010015

Figure 2.

Figure 2

Antibacterial activity of CAM-Cn-BER, n = 1, 2, 3, 5, 8. Levofloxacin (LEV), chloramphenicol (CHL), and erythromycin (ERY) are used as the controls. (A) Testing of the CAM-Cn-BER activity using E. coli ΔtolC pDualrep2 reporter strain. The induction of the red fluorescent protein expression (green halo around the inhibition zone, pseudocolor) is triggered by DNA damage, while the induction of Katushka2S protein (red halo, pseudocolor) occurs in response to ribosome stalling. The unlabeled spot above LEV corresponds to a substance not studied in this work. (B) Testing of the CAM-Cn-BER antibacterial activity on CHL-resistant E. coli strain ΔtolC-CAT harboring pCA24N-LacZ plasmid with the cat gene encoding for chloramphenicol acetyltransferase. (C) Testing of the CAM-Cn-BER antibacterial activity on macrolide-resistant E. coli strain SQ110 ΔtolC (A2058G) in which the A2058 nucleotide is replaced by G2058 in the 23S rRNA.