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(A) Schematics of the integration of suicide plasmid pBFS-57 into the bspA locus of the B. forsythus genome. (B) Southern hybridization. Genomic DNA isolated from B. forsythus wild-type (ATCC 43037, lanes 1, 4, and 7), mutant (BFM-571, lanes 2, 5, and 8), and suicide plasmid DNA (pBFS-57, lanes 3, 6, and 9) digested with restriction enzymes shown on top were hybridized with the bspA probe. Numbers on the left indicate sizes in kilobases.
