FIG. 3.

Western immunoblot analysis of wild-type and mutant B. forsythus. Proteins were transferred onto nitrocellulose membranes after separation on an SDS–10% PAGE gel. Membranes were probed with anti-rBspA antibody (16). Lanes: 1, total cell lysate of wild-type B. forsythus ATCC 43037; 2, total cell lysate of mutant BFM-571; 3, recombinant BspA protein (70-kDa rBsp70). The positions of molecular mass standards (in kilodaltons) are shown on the left.