Figure 5.

Immunohistochemical staining and quantification of active caspase-3 in rat liver after DF treatment and OR, Bet and NAC treatments and pretreatments in rat livers. (A) Representative tissue sections are shown. 400×. Scale bar = 30 µm. (B,C) Photographs of 18–22 fields (1.25 mm²) per group were taken, and the number of positive hepatocytes was determined, in addition to the intensity of the reaction area, using NIH ImageJ ver 2.3.0/1.530 software. Bar graphs show the mean (n = 4) ± SEM. Control group. p > 0.05, ***: p ≤ 0.001, ****: p ≤ 0.0001 vs. Control group. ####: p ≤ 0.0001 vs. DF group.