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. 2022 Nov 14;64(1):91–98. doi: 10.1093/jrr/rrac077

Fig. 1.

Fig. 1

The expression status of LAT1 in the cells used in this study. (A) Schematic diagram of the Slc7a5 locus, 6 × His-tag, and the targeted site of CRISPR/Cas9 for the generation of SCC7-ΔLAT1. (B) Schematic diagram of the Rosa26 locus for the generation of SCC7_LAT1_enhance. (C) Immunoblot analysis of SCC7-ΔLAT1. Con (control): SCC-WT cells before the generation of SCC7-ΔLAT1 cells by CRISPR/Cas9, DLAT1: SCC7-ΔLAT1 cells. (D) CBB stain, immunoblot and RT-PCR results of SCC7_LAT1_enhance. Con (control): SCC-WT cells transfected with an empty vector containing only a neomycin-resistance cassette (PGK-neo), Enh: SCC7_LAT1_enhance cells. A P value of less than 0.05 were considered to be statistically significant (****P < 0.0001).