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. 2022 Dec 30;132(2):208–222. doi: 10.1161/CIRCRESAHA.122.321153

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© 2023 The Authors.

Circulation Research is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 5. — Knockout of ASPP1 improves cardiac I/R injury. A, Verification of ASPP1 conventional knockout in mice by Western blot (Student t test); n=6. B, Cardiac function of wild type (WT) and ASPP1 knockout (KO) mice after I/R injury by echocardiography (2-way ANOVA, followed by Tukey post hoc multicomparisons test); n=11. C, Infarct area of I/R mice by 2,3,5-triphenyltetrazolium chloride (TTC) staining (Mann-Whitney U test); n=15. Scale bar=2 mm. D and E, Serum LDH (Kruskal-Wallis, followed by false discovery rate [FDR] method of Benjamini and Hochberg test) and creatine kinase isoenzyme MB (CKMB; 2-way ANOVA, followed by Tukey post hoc multicomparisons test) levels; n=13 (WT+Sham and ASPP1[KO]+Sham); n=15 (WT+I/R and ASPP1[KO]+I/R) for LDH assay; n=9 for CKMB assay. F, Caspase-3 activity detected by ELISA assay (2-way ANOVA, followed by Tukey post hoc multicomparisons test); n=9. G and H, The protein levels of Bcl2 and Bax detected by Western blot (2-way ANOVA, followed by Tukey post hoc multicomparisons test); n=6. I, Immunofluorescent staining was performed to analyze the effects of ASPP1 knockout on p53 nuclear translocation in isolated adult cardiomyocytes after cardiac I/R injury (Kruskal-Wallis, followed by FDR method of Benjamini and Hochberg test); n=5; ns, not significant. Scale bar=20 μm. ASPP1 indicates apoptosis stimulating of p53 protein 1; DAPI, 4′,6-diamidino-2-phenylindole; I/R, ischemia/reperfusion; and LDH, lactate dehydrogenase.