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. 2022 Dec 30;132(2):208–222. doi: 10.1161/CIRCRESAHA.122.321153

Figure 6.

Figure 6.

Knockdown of ASPP1 alleviates H/R-induced damage in cultured neonatal mouse ventricular cardiomyocytes (NMVCs). A, Efficiency of small interfering RNA (siRNA) for ASPP1 in NMVCs by Western blot (Mann-Whitney U test); n=5. B, LDH release (1-way ANOVA, followed by Tukey post hoc multicomparisons test); n=6. C, Apoptosis of NMVCs detected by TUNEL (TdT-mediated dUTP nick end labeling) assay (1-way ANOVA, followed by Tukey post hoc multicomparisons test); n=6. Scale bar=50 μm. Green, TUNEL - positive cells; blue, DAPI. D, Mitochondrial membrane potential of NMVCs by JC-1 assay (1-way ANOVA, followed by Tukey post hoc multicomparisons test); n=6. Scale bar=50 μm. E, Caspase-3 activity in NMVCs by ELISA assay (1-way ANOVA, followed by Tukey post hoc multicomparisons test); n=6. F and G, The protein levels of Bcl2 and Bax detected by Western blot (Kruskal-Wallis, followed by false discovery rate [FDR] method of Benjamini and Hochberg test); n=5. ASPP1 indicates apoptosis stimulating of p53 protein 1; Bax, Bcl2-associated X; DAPI, 4′,6-diamidino-2-phenylindole; H/R, hypoxia/reoxygenation; and LDH, lactate dehydrogenase.