Table 25.
Demonstrations of SiP biosensors using lipid nanodiscs as the biorecognition element and their sensing performance. All tabulated demonstrations used a label-free assay format.
| Bioreceptor | Sensor Type | Target | Detection Performance | Ref. | |
|---|---|---|---|---|---|
| Figure of Merit | Value | ||||
| Lipid nanodiscs containing PC, four binary compositions of PC and PS, and two binary combinations of PS and PA | Si MRR | Blood clotting proteins: pro-thrombin, factor X, activated factor VII, and activated protein C | - | - | [149] |
| Lipid nanodiscs containing POPC and POPC/POPS | Si MRR | Annexin V | - | - | [150] |
| Lipid nanodiscs containing GM1 | Si MRR | Cholera Toxin Subunit B | - | - | |
| Lipid nanodiscs containing biotin-DPPE | Si MRR | Streptavidin | - | - | |
| Lipid nanodiscs containing CYP3A4 | Si MRR | Anti-CYP3A4 antibody | - | - | |
| Lipid nanodiscs with 9 different compositions containing PS, PE, and PC. | Si MRR | Protein clotting factors: prothrombin, activated factor VII, factor IX, factor X, activated protein C, protein S, and protein Z | - | - | [148] |
PC: phosphatidylcholine, PS: phosphatidylserine, PA: phosphatidic acid, POPC: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, POPS: 1-palmitoyl-2-oleoyl-sn-glycero-3-(phospho-L-serine), GM1: monosialotetrahexosyl ganglioside, biotin-DPPE: N-(biotinoyl)-1,2-dipalmitoyl-sn-glycer-3-phosphoethanolamine, CYP3A4: cytochrome P450 3A4, and PE: phosphatidylethanolamine.