Table 32.
Strategies demonstrated for the immobilization of glycans and lectins on SiP sensors and representative surfaces.
| Glycan and Lectin Immobilization | ||||||
|---|---|---|---|---|---|---|
| Immobilization Strategy | Bioreceptor Subtype | Surface Pre-Treatment | Chemical Surface Modification | Linking Strategy | Sensor Type | Refs. |
| Covalent | Glycan | Piranha treatment | UDPA organophosphonate surface modification | DVS activation | Si MRR | [126] |
| Covalent | Glycan | Piranha and UV/ozone plasma treatment | MPTMS silanization | SM(PEG)12 linker | Si3N4 MRR | [133] |
| Covalent | Glycan | Piranha treatment | APTES silanization | BS(PEG)9 linker | Si3N4 MRR | [132] |
| Covalent | Lectin | Hydrogen peroxide and thermal treatment | APTES silanization | Glutaraldehyde linker | Porous Si sensor | [131] |
| Non-covalent | Glycoproteins and neoglycoconjugates | - | - | Passive adsorption | Si MRR | [246] |
| Covalent + bioaffinity | Lectin (biotinylated) | UV/ozone clean | APTMS silanization | NHS-PEG4-biotin linker + avidin | Si3N4 reflectometric interference spectroscopy sensor | [285] |
SM(PEG)12: succinimidyl-([N-maleimidopropionamido]-dodecaethyleneglycol) ester, BS(PEG)9: bis-N-succinimidyl-(nonaethylene glycol) ester, NHS-PEG4-biotin: N-hydroxysuccinimide ester-polyethylene glycol-biotin.