Figure 3.

CBD decreased expression of inflammatory markers in intestinal organoids. Mature ileal organoids were treated with Tnfα + LPS (TL) to induce inflammation or treated with increasing concentrations of CBD in the absence or presence of TL. Negative controls consisted of no treatment (NT). On day 4, after organoids were passaged, organoids were incubated with treatments (n = 6 wells per treatment) for 24 h; each well contained approximately 100 mature organoids. Organoids were harvested and RNA was extracted for qPCR and the relative expression of target genes were determined using the 2e-Δct method. Outliers were not detected after ROUT test. Significant differences between treatments were detected using one-way ANOVA followed by a Tukey post hoc test. Different letters indicated significant difference between treatments.