Table 1.
Sequence and GenBank accession numbers of oligonucleotides used in RT-qPCR.
| Target | GenBank Accession Number | Forward Reverse |
Sequence (5′→3′) |
|---|---|---|---|
| Tnfα | NM_000594 | Forward Reverse |
TCCTTCAGACACCCTCAACC AGGCCCCAGTTTGAATTCTT |
| Il1β | NM_000576 | Forward Reverse |
GGGCCTCAAGGAAAAGAATC TTCTGCTTGAGAGGTGCTGA |
| Il6 | NM_000600 | Forward Reverse |
TACCCCCAGGAGAAGATTCC TTTTCTGCCAGTGCCTCTTT |
| Il10 | NM_000572 | Forward Reverse |
GCCTAACATGCTTCGAGATC TGATGTCTGGGTCTTGGTTC |
| Ccr7 | NM_007719.2 | Forward Reverse |
GTGTGCTTCTGCCAAGATGA CCACGAAGCAGATGACAGAA |
| iNos | NM_ 000625 | Forward Reverse |
ACCCAGACTTACCCCTTTGG GCCTGGGGTCTAGGAGAGAC |
| Arg1 | NM_007482.3 | Forward Reverse |
CGCCTTTCTCAAAAGGACAG ACAGACCGTGGGTTCTTCAC |
| Ym1 | NM_009892.3 | Forward Reverse |
ACTTTGATGGCCTCAACCTG AATGATTCCTGCTCCTGTGG |
| Nlrp3 | NM_001359638.1 | Forward Reverse |
AAGCAACAGATGGAGACCGG CAAATTCCATCCGCAGCCAG |
| Asc | NM_023258.4 | Forward Reverse |
GTCTTAGGGGCGGAAACCAA CCGCGGTCACCTTTTACTCT |
| Casp1 | NM_009807.2 | Forward Reverse |
ACTGACTGGGACCCTCAAGT AACTTGAGCTCCAACCCTCG |
| Il18 | NM_001357221.1 | Forward Reverse |
TCGCAGCAGGGTTTTCTAGG ACGGGAGGGAGAAAGACTGA |
| Hprt | NM_001289746 | Forward Reverse |
ACCCCACGAAGTGTTGGATA AAGCAGATGGCCACAGAACT |
| Gapdh | NM_001289726 | Forward Reverse |
AACTTTGGCATTGTGGAAGG ACACATTGGGGGTAGGAACA |