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. 2023 Jan 7;13(1):123. doi: 10.3390/biom13010123

Figure 4.

Figure 4

Loss of miR-146a in chondrocytes protects against injury-induced OA in mice. (a) Schematic illustration of in vivo experimental design; (b) Safranin O/fast green staining of knee sections of miR-146af/f (control) and Agc1CreERT2;miR-146af/f (miR-146aAgc1, miR-146a LOF) mice at 10 weeks following MLI surgery (n = 7) (scale bar, 100 μm); (c) MicroCT images of subchondral bone from control and miR-146a LOF mice at 10 weeks post-MLI-surgery (n = 7) (scale bar, 0.5 mm); (d) Quantifications of bone volume over total tissue volume (BV/TV) in reconstructed subchondral bone from control and miR-146a LOF mice at 10 weeks following MLI (n = 7); (e) Osteoarthritis Research Society International (OARSI) scores for the medial tibial plateau and femoral condyle from control and miR-146a LOF mice at 10 weeks following MLI (n = 7); OA-related pain assessed by (f) electronic Von Frey and (g) Small Animal Algometer (SMALGO) tests in control and miR-146a LOF mice at 10 weeks following MLI (n = 7). Data presented as mean ± SD. * p < 0.05 by Student’s t-test; (h) IHC for phospho-p65, Mmp13, and Prg4 on knee sections of control and miR-146a LOF mice at 10 weeks post-MLI (n = 7) (scale bar, 100 μm).