TABLE 2.

Primers used for this study

Primer	Primer sequence	Comment
1003	5′-ACGGGGATCCTTATGACGTTGGAGAAAA	Upstream of sarT, BamHI site added (underlined)
1004	5′-AGCGGGGATCCCAAGTTTTACCAGCATA	Downstream reverse primer, sarT
1005	5′-GTAAGGGATGAACTCGAGATGAATGATT	Start of sarT (bold), added XhoI site (underlined)
1006	5′-ACGGGGATCCAAAAATACATTTAACTGC	Reverse primer, downstream of sarT, BamHI site added (underlined)
1013	5′-ATGGTCTATTTCAATGGCAGTTAC-3′	Internal reverse primer, ermC
1017	5′-GATGCGATTGAACGTATGAATAATGAT-3′	Upstream of primer 1003
1035	5′-GCGAATTCTACCGGTCCTTTCTTATCTCT	Downstream of sarT coding for complete transcript, EcoRI site added (underlined)
1036	5′-GCGAATTCCAGATTGTTTGTAAAGTATGT	Upstream sarT complete transcript, EcoRI site added