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. 2023 Jan 13;13(1):136. doi: 10.3390/bios13010136

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Schematic of the step-by-step process of PDMS device fabrication. (Step 1) The master mould is created using soft lithography. (Step 2) The PDMS mixture (10:1 (base: curing agent)) is poured over the master mould and cured. (Step 3) The solidified PDMS is then cut and peeled from the master mould. (Step 4) The inlets and outlets are punched using a biopsy puncher. (Step 5) The PDMS chip and glass slide/coverslip are activated through plasma treatment. (Step 6) Finally, the PDMS chip is bound to a glass slide/coverslip and cured further for ~2–5 min at 75 °C and is ready to use.