Table 3.
Primers used for quantitative Real-Time PCR analysis of human and murine genes.
| Gene | Reference Number | Forward Sequence (5′-3′) |
Reverse Sequence (5′-3′) |
Species |
|---|---|---|---|---|
| IL-8 | NM_001310420.1 | CTCTTGGCAGCCTTCCTGAT | TTTGGGGTGGAAAGGTTTGGA | Human |
| IL-18 | NM_001386420.1 | AAGATGGCTGCTGAACCAGT | TGCCAAAGTAATCTGATTCCAGG | Human |
| IL-1β | NM_000576.3 | TCGCCAGTGAAATGATGGCT | GGTCGGAGATTCGTAGCTGG | Human |
| Cyclin D1 | NM_053056 | AGCTGTGCATCTACACCGAC | GAAATCGTGCGGGGTCATTG | Human |
| MAPK1/ERK | NM_002745.5 | CGTGTTGCAGATCCAGACCA | GCCAGAATGCAGCCTACAGA | Human |
| β-Actin | NM_001101.5 | ACAGAGCCTCGCCTTTGC | CGCGGCGATATCATCATCCA | Human |
| B2M | NM_004048.4 | CTGCCGTGTGAACCATGTGA | TCAAACCTCCATGATGCTGC | Human |
| HPRT | NM_000194.3 | TGCTGAGGATTTGGAAAGGGT | GGGCTACAATGTGATGGCCT | Human |
| IL-8/CXCL8 | NM_011339.2 | TGATGCTCCATGGGTGAAGG | CAGAAGCTTCATTGCCGGTG | Murine |
| IL-18 | NM_001357221.1 | GGCTGCCATGTCAGAAGACT | ACAGTGAAGTCGGCCAAAGT | Murine |
| IL-1β | NM_008361.4 | GCCACCTTTTGACAGTGATGAG | GACAGCCCAGGTCAAAGGTT | Murine |
| Cyclin D1 | NM_001379248.1 | AAACAAGGACCCCCTCCATC | GGCTTCAATCTGTTCCTGGC | Murine |
| MAPK1/ERK | NM_001038663.1 | CCTCCTGCTGAACACCACTT | ATCTGGATCTGCAACACGGG | Murine |
| β-Actin | NM_007393.5 | CTGTCGAGTCGCGTCCACC | CGCAGCGATATCGTCATCCAT | Murine |
| B2M | NM_009735.3 | GAGCCCAAGACCGTCTACTG | GGTTCAAATGAATCTTCAGAGCATC | Murine |
| HPRT | NM_013556.2 | TTCTTTGCTGACCTGCTGGA | TTATGTCCCCCGTTGACTGA | Murine |