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Representative fluorescent micrographs of whole-mount preparations of myenteric ganglia from the duodenum (a), ileum (b) and colon (c) of a diabetic rat after TLR4-peripherin double-labelling immunohistochemistry. Peripherin was used as a pan-neuronal marker to label myenteric neurons. Arrows—TLR4-immunoreactive myenteric neurons, arrowheads—myenteric neuron, scale bars: 20 μm.
