TABLE 1.
Serum IgG antibody reactive with HDS or S. mutans GTF after i.n. administration of PLGA-HDS in CT or detoxified E. coli enterotoxin (dLT)
| Antigen (expt no.) | Adjuvant | Mean serum IgG EU ± SE (P)b reactive with:
|
||||
|---|---|---|---|---|---|---|
| HDS
|
S. mutans GTF
|
|||||
| 1° responsea | 2° response | 3° response | 1° response | 3° response | ||
| PLGA (3) | CT | <1 | <1 | <1 | 9 ± 3 | 7 ± 1 |
| PLGA-HDS (3) | CT | 70 ± 17 (<0.001) | 109 ± 12 (<0.001) | 165 ± 9 (<0.001; <0.001) | 25 ± 10 (<0.03) | 66 ± 43 (<0.04) |
| PLGA-HDS (4) | dLT | 46 ± 16 (<0.001) | 153 ± 7 (<0.001; <0.001) | 175 ± 3 (<0.001; <0.001) | 19 ± 6 (<0.05) | 123 ± 48 (<0.001) |
a
Samples were collected on days 24, 112, and 211 for the CT groups and on days 26, 110, and 188 for the dLT group.
b
P indicates the level of significance (one-way ANOVA) compared to the control group serum response on the same day. Where two P values are given, the second value indicates the level of significance (one-way ANOVA) compared to the respective immune group serum response of the previous collection period.