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. 2023 Jan 5;12(2):224. doi: 10.3390/cells12020224

Figure 2.

Figure 2

Timeline of p38/MK2/HSP27 activation in hASCs and hBMSCs. (A,B) hASCs and hBMSCs were cultured in BM, BaG BM, OM, and BaG OM for 1, 3, 5, 7, 9, and 11 days, and analyzed with Western Blotting and immunodetection. The figure presents cropped blots of p-p38, p38, pMK2, MK2, pHSP27(S78), HSP27, and β-actin of the representative donor cell lines. Full-length blots are presented in Figure S4. (C,D) Semi-quantification of the pHSP27(S28) bands presented normalized to β-actin in hASCs and hBMSCs. Abbreviations basic medium, BM; osteogenic medium, OM; and bioactive glass, BaG.