Table 1.

Primary and secondary antibodies used in immunodetection.

Antibody Type	Antibody	Host Species	Dilution	Incubation
Primary	anti-β-actin sc-47778 †	mouse	1:2000	RT, 2 h
Primary	anti-HSP27 (D6W5V) #95357S ‡	rabbit	1:1000	+4 °C, overnight
Primary	anti-p-HSP27(S78) #2405S ‡	rabbit	1:1000	+4 °C, overnight
Primary	anti-MAPKAPK2 #12155T ‡	rabbit	1:1000	+4 °C, overnight
Primary	anti-p-MAPKAPK2 (T334) #3007T ‡	rabbit	1:1000	+4 °C, overnight
Primary	anti-p38α sc-728 †	rabbit	1:100	+4 °C, overnight
Primary	anti-p-p38 MAPK(T180/Y182) #4511S ‡	rabbit	1:1000	+4 °C, overnight
Secondary	anti-mouse IgG-HRP (sc-2005) †	goat	1:2000	RT, 1h
Secondary	anti-rabbit IgG-HRP #7074S ‡	goat	1:2000	RT, 1h

_† Santa Cruz Biotechnology, Dallas, TX, USA. _‡ Cell Signaling Technology, Danvers, MA, USA.