Table 2.
Reagents used in ICC staining.
| Antibody Type | Antibody | Host Species | Dilution | Incubation |
|---|---|---|---|---|
| Primary | anti- collagen type I (ab90395) † | mouse | 1:2000 | +4 °C, overnight |
| Primary | anti-pHSP27 (S78) (ab32501) † | rabbit | 1:500 | +4 °C, overnight |
| Primary | anti-HSP27 (D6W5V) #95357S ‡ | rabbit | 1:500 | +4 °C, overnight |
| Secondary | anti-mouse IgG Alexa fluor 488 (A11029) § | goat | 1:500 | +4 °C, 45 min |
| Secondary | anti-rabbit IgG Alexa fluor 488 (A21206) § | donkey | 1:500 | +4 °C, 45 min |
| - | Phalloidin-TRITC § | - | 1:500 | +4 °C, 45 min |
| - | DAPI § | - | 1:2000 | RT, 5 min |
† Abcam, Cambridge, United Kingdom. ‡ Cell Signaling Technology, Danvers, MA, USA. § Sigma-Aldrich, Saint Louis, MO, USA.