Table 3.
Antigen | Surface Protein | Surface Marker Expression hASC (%) | Surface Marker Expression hBMSC (%) | Fluorophore | Manufacturer |
---|---|---|---|---|---|
CD11a | Integrin alpha L (Lymphocyte function-associated antigen 1) | 1.5 ± 0.7 | 0.7 ± 0.4 | APC | R&D Systems Inc. Minneapolis. MN. USA |
CD14 | Lipopolysaccharide receptor | 1.2 ± 0.9 | 5.4 ± 1.8 | PECy7 | BD Biosciences. Franklin Lakes. NJ. USA |
CD19 | B lymphocyte-lineage differentiation antigen | 1.1 ± 0.9 | 4.3 ± 2.4 | PECy7 | BD Biosciences |
CD34 | Hematopoietic progenitor cell antigen 1 | 22.2 ± 23.4 | 2.5 ± 0.5 | APC | Immunotools GmbH. Friesoythe. Germany |
CD45 | RO isoform of leucocyte common antigen | 1.8 ± 0.3 | 7.1 ± 1.4 | APC | BD Biosciences |
CD73 | Ecto-5′-nucleotidase | 91.1 ± 3.7 | 93.4 ± 3.7 | PE | BD Biosciences |
CD90 | Thy-1 (T cell surface glycoproteins) | 98.8 ± 1.0 | 88.6 ± 6.5 | APC | BD Biosciences |
CD105 | SH-2. Endoglin | 94.7 ± 6.9 | 92.9 ± 6.1 | PE | R&D Systems Inc. |
HLA-DR | Major histocompatibility class II antigen (MHC-II) | 1.3 ± 1.1 | 85.7 ± 8.3 | PE | Immunotools GmbH |
Abbreviations: human adipose stem cell, hASC; human bone marrow stem cell, hBMSC; cluster of differentiation, CD; allophycocyanin, APC; phycoerythrin-cyanine, PECy7; phycoerythrin, PE. Standard deviation is indicated with ±.