Figure 7.

Effects of platelet-derived mEVs on the expression of COX-2 (PTGS2) in cocultures with cancer cells. Cancer cells (0.5 × 10⁶), (A) (HCA7 cells), (B) (HCT116 cells), (C) (HT29 cells), and (D) (Caco2 cells) were incubated for 4 and 24 h with EVs from CRC or HS individuals (100 μg), and the gene expression of PTGS2 was evaluated by qPCR and normalized to that of GAPDH and reported as fold-change vs. the gene expression detected in the cocultures of mEV from HS with the cancer cells. The values were not normally distributed and were transformed to logarithms, and all values are shown as scatter dot plots with mean + SD, n = 10, and analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. (A) 24 h, ** p < 0.01 vs. vehicle and EV HS. (B) 24 h, ** p < 0.01 vs. vehicle and EV HS. (C) 4 h,** p < 0.01 vs. vehicle and EV HS; 24 h, # p < 0.01 vs. vehicle and EV HS. (D) 24 h, * p < 0.01 vs. vehicle and EV HC.