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. 2023 Jan 6;12(2):242. doi: 10.3390/cells12020242

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Confocal images of immunostaining of collateral dependent zone (CZ) of GFP⁺CD34⁺ BMT recipient rat heart after 10 days’ repetitive ischemia (RI). (a) GFP⁺CD34⁺ BMSCs colocalized with endothelial cell (EC) marker isolectin-B4 (as shown by arrows), indicating that GFP⁺CD34⁺ BMSCs differentiated into EC during coronary collateral growth (CCG) induced by RI. (b,c) GFP⁺CD34⁺ BMSCs colocalized with EdU (as shown by arrows), suggesting that GFP⁺CD34⁺ BMSCs proliferated during CCG. The scale bar equals 10 μm.