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. 2023 Jan 5;15(2):362. doi: 10.3390/cancers15020362

Figure 3.

Figure 3

Generation of a specific anti-STARD3 antibody for immunohistochemistry. (A) Western blot analysis of STARD3 expression using the anti-STARD3 antibody 3G11 in HCC1954 cells (WT and transfected with control (siCtrl) and STARD3-targeting (siSTARD3)-siRNAs). Anti-Rab7 antibody was used as a loading control. (B) Western blot analysis of HeLa cells transiently expressing STARD1, STARD3, STARD4, STARD5, and STARD6 fused to GFP (GFP-STARD) using anti-STARD3 3G11 antibody. Anti-Rab7 antibody was used as a loading control. (C) HCC1954 cells (WT) and HCC1954 cells transfected with control siRNAs (siCtrl), and siRNAs targeting STARD3 (siSTARD3) were labeled with anti-STARD3 3G11 antibodies (red). Nuclei are stained blue. Scale bar: 10 μm. (D) Immunohistochemistry on normal breast (a) and HER2+ breast tumor (b) sections using anti-STARD3 3G11 antibody. N marks the position of a normal duct. S marks the tumor stroma. The subpanel (c) is a higher magnification image (4.5×) of the area outlined in black in (b). Scale bar: 50 μm (a,b) and 10 μm (c). All the whole western blot figures can be found in the Supplementary Materials.