Figure 1.

HNSCC tumor slices remain viable during five days of culture. (A) Representative H&E staining at day 0 (start of the culture), day 2, and day 5 of ex vivo culture. (B) Representative microscopy images of TUNEL staining at days 0 and 5 of ex vivo culture. (C) Quantification of TUNEL-positive at the start, and after 5 days of ex vivo culture. Graph bars represent the mean of ≥5 FoV per sample, and error bars show SEM. A t-test was used to assess significance. ** p < 0.01.