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. 2023 Jan 5;12(2):226. doi: 10.3390/cells12020226

Figure 10.

Figure 10

Big tau expression after SCG explant lesion (5 days). P3 SCG explants were cultured for 8 days and then continued to grow for 5 days after lesion. Panels (A–D) show SCG explant with lesion at low magnification. Panels (E–H) show SCG neurites growing at the high magnification. The DAPI staining show the explant cell bodies (A,E), while Tuj (B,F) and Big tau (C,G) antibodies also stain the regrowing neurites (merged in (D,H)). Note that at 5 days post-lesion Big tau is expressed along the entire length of the neurites similar to the staining of Tuj. The analysis of the ratio of Big tau/Tuj 5 days after lesion is based on data on presented in (B,C). Panels (I,J) indicate the proximal and distal locations (n = 3) of the areas included in average density calculation, respectively, expressed as a ratio of Big Tau over Tuj (K). There is no significant difference between the ratios of Big tau/Tuj densities at proximal explant relative to distal (p = 0.22). Scale bar = 100 µm.