Figure 4.

Changes of translation reinitiation and termination in arsenite-treated wt and ddG3BP1/2 U2OS cells. Wt (A) and ddG3BP1/2 (B) U2OS cells were transfected with the same reporter mRNAs as in Figure 1. Two hours after transfection, cells were lysed, and luciferase activities were measured and normalized, as described above. Translation efficiencies of the reporter mRNAs were normalized by dividing by the translation efficiencies of the corresponding control transcripts, in untreated or 150 µM arsenite-treated cells. The mean values (±SD) of at least three independent experiments are shown. The absolute values were roughly similar to those indicated in the legend in Figure 1 (and were at least two orders of magnitude above the background).