Table 1.
Localization of translation machinery components in SGs.
| Cellular Component | Localization in Mammalian SGs | References | Remarks | |
|---|---|---|---|---|
| poly(A)+ mRNA |
Yes | [29] | ||
| 40S | Yes | [29] and many more | ||
| 60S | No | [29,42,43] | ||
| PABP | Yes | [44] and many more | ||
| Translation initiation | eIF1 | No data | In [45] eIF1 was mentioned as detected in SGs (as an unpublished observation of the authors). | |
| eIF1A | No data | Yeast eIF1A localizes to SGs under certain, but not all, stress conditions [33,46,47]. | ||
| eIF2 | Diverse or controversial | [29,43,48] | “Canonical” SGs lack eIF2, although under some conditions eIF2 was found in SGs (for review, see [8]). | |
| eIF2B | Diverse or controversial | [29,43] | Yeast eIF2B was reported to reside in large cytoplasmic foci even in the absence of stress [49]. | |
| eIF3 | Diverse | [29] and many more | eIF3 is absent in UVC- or selenite-induced SGs [32,50] but present in the “canonical” SGs (for review, see [8]). | |
| eIF4A | Yes | [23,25,30,32,48] | Two paralogs (eIF4A1 and eIF4A2), encoded by different genes are both localized to SGs. | |
| eIF4E | Yes | [29] and many more | ||
| eIF4G | Yes | [23,29,32] | Two paralogs (eIF4GI and eIF4GII), encoded by different genes, are both localized to SGs. | |
| eIF4B | Yes | [48,51] | ||
| eIF4H | Yes | [25] | ||
| eIF5 | No | [29] | ||
| eIF5B | No data | Yeast eIF5B localizes to NaN3-induced SGs [47]. | ||
| eIF2A | Controversial | [23,52] | According to mass-spectrometry data, eIF2A associates with SG; however, immunofluorescence data contradicts this. | |
| Elongation | eEF1A | No data | Yeast eEF1A is excluded from the heat-shock induced SGs [33]. | |
| eEF1B | No data | Yeast eEF1Bγ is present in the heat-shock induced SGs [33]. | ||
| eEF2 | No | [43] | Yeast ortholog of eEF2 (Eft1p) was reported to absent from SGs [33]. | |
| eIF5A | Yes | [31,32] | ||
| Termination | eRF1 | This study | Yeast eRF1 (Sup45p) was reported to localize to heat-shock induced SGs [33]. | |
| eRF3 | This study | Yeast eRF3 (Sup35p) was reported to localize to heat-shock induced SGs [33]. | ||
| Recycling | ABCE1 | This study | No information about localization of the yeast orthologs (Rli1p, Tma64p, Tma20p, and Tma22p, respectively) is available as well | |
| eIF2D | Yes & This study | [52] | ||
| MCT-1 | This study | |||
| DENR | This study |