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NanoBRET in-cell target engagement of CAMKK2 probe (5) (n = 3) and STO-609 (n = 1) in HEK293 cells transiently transfected with NLuc-CAMKK2. Increasing doses of inhibitor displaces the fluorescent inhibitor ligand (called a tracer) used in the assay, leading to a reduction in the BRET signal.
