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. 2023 Jan 16;15(2):546. doi: 10.3390/cancers15020546

Figure 1.

Figure 1

Inhibition of DHODH suppresses cell proliferation and promotes cell death in cervical cancer cells. (A) The expression level of DHODH protein in human cervical cancer tissues and adjacent non-tumor tissues was measured by IHC staining. Scale bar = 100 μm. IHC scores were used to analyze the experimental results. *** p < 0.001 compared to the normal tissues. (B) The efficacy of DHODH knockdown in CaSki and HeLa cells was measured by Western blotting. The relative expression of DHODH was calculated by ImageJ software in each group (n = 3). ** p < 0.01, *** p < 0.001 compared to the control group. (C) The effect of DHODH knockdown on cell viability was measured by CCK-8 assays in cervical cancer cells. *** p < 0.001 compared to the control group. (D) The effect of DHODH knockdown on clonogenicity was measured by the colony formation assay in cervical cancer cells. (E) Sensitivity to brequinar (BQR) was assessed using the CCK-8 assay in CaSki and HeLa cells treated with BQR (0, 0.01, 0.1, 1, 10, 100 μM) for 24, 48 and 72 h. (F) The effect of BQR (0.7 μM for CaSki cells; 0.3 μM for HeLa cells) on cell death was assessed by the flow cytometry analysis through PI staining. *** p < 0.001 compared to the control group.