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. 2023 Jan 16;15(2):546. doi: 10.3390/cancers15020546

Figure 2.

Figure 2

Inhibition of DHODH induces ferroptotic cell death in cervical cancer cells. (A) The effect of DHODH knockdown on lipid peroxidation in CaSki and HeLa cells was measured by MDA level detection. ** p < 0.01, *** p < 0.001 compared to the control group. (B) The effect of brequinar (BQR) on lipid peroxidation in CaSki (2 μM) and HeLa (1 μM) cells was measured by MDA level detection after treated for 48 h. *** p < 0.001 compared to the control group. (C) Cell viability of CaSki and HeLa cells treated with BQR (0.7 μM for CaSki cells; 0.3 μM for HeLa cells) and/or liproxstatin-1 (Lip-1) (2 μM) for 24 h was measured by CCK-8 assays. * p < 0.05, ** p < 0.01, *** p < 0.001. (D) The lipid peroxidation of CaSki and HeLa cells treated with BQR (0.7 μM for CaSki cells; 0.3 μM for HeLa cells) and/or Lip-1 (2 μM) for 24 h was measured by MDA level detection. ** p < 0.01, *** p < 0.001. (E) The mitochondrial dysfunction in CaSki and HeLa cells treated with or without BQR (0.7 μM for CaSki cells; 0.3 μM for HeLa cells) was measured by mitochondrial membrane potential detection through JC-1 staining. Scale bar = 100 μm. *** p < 0.001 compared to the control group.