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. 2023 Jan 9;12(2):264. doi: 10.3390/cells12020264

Figure 3.

Figure 3

Acute effects of TC-G 1008 (TC-G) (20 mg/kg), ZnCl2 (16 mg Zn/kg), or VPA (150 mg/kg) and MES seizures on the relative expression of proteins: phosphorylated CREB (p-CREB), total CREB, BDNF, phosphorylated TrkB (p-TrkB) and TrkB in the hippocampi of mice fed the ZnA or ZnD diet. Hippocampal samples were obtained ca. 3 min after MES. (A–F) Data are expressed as means ± SEM of p-CREB/CREB or BDNF/β-actin or p-TrkB/TrkB ratio. They were analyzed by one-way ANOVA. n = 4–6 in each group. Statistical details: (A) F(4,23) = 1.100, p = 0.3805, (B) F(4,23) = 0.6353, p = 0.6425, (C) F(4,23) = 0.5934, p = 0.6709, (D) F(4,23) = 1.218, p = 0.3306, (E) F(4,23) = 1.100, p = 0.3805, (F) F(4,23) = 0.6353, p = 0.6425 (A-F one-way ANOVA). (G) Representative blots of p-CREB (Ser 133), CREB (~46 kDa), BDNF (~14 kDa), p-TrkB (Tyr 816), TrkB (~140 kDa) and β-actin (~42 kDa) in the hippocampi of mice. #p-CREB and p-TrkB come from the same blot, thus sharing the corresponding β-actin band.