Figure 1.

Influence of the orb2 3’UTR deletion on fly locomotion and memory formation. (A) Schematic representation of orb2 mRNAs. The vertical-colored lines in the 3′UTR (gray region) show the CPE sequences. Different colors of lines correspond to different CPE sequences (see [26] for details). The red dashed lines show the deleted part of the 3′UTR. (B,C) The percentage of WT and orb2^R flies that could climb after strong shaking (climbing assay, B) and restore the normal body position after intense shaking (vortex assay, C). The standard deviation is shown, t-test, n = 100; ns – nonsignificant (D,E) Learning indices of WT and orb2^R flies as calculated for short-, mid- (D), and long-term (E) memory by assessing the suppression of male sexual behavior after interaction with a previously fertilized female. Assessment of differences between learning indices based on calculated courtship indices (two-side randomization test, p < 0.05, n = 20).