Figure 2.

Effect of the 3′UTR deletion on orb2 gene expression in the brain. (A) Relative levels of the orb2 mRNA in the heads of WT and orb2^R flies as determined by qPCR. ns - nonsignificant. (B) Relative amount of the Orb2 protein in the heads of WT and orb2^R flies as detected by semiquantitative Western blot. Error bars show the standard deviation (t-test, n = 5). (C) Western blot analysis of head lysates of WT and orb2^R flies with antibodies against the Orb2 protein (top). Total protein staining (below) was used for normalization in two-fold serial dilution.