Figure 7.

Collision of the suprathreshold spikes. (A) Both suprathreshold spikes and subthreshold waves were triggered in the temporal area of the slice or initiated in the septal area of the slice. (B) The constructive interference can only be observed outside the window of the suprathreshold spike duration. (C) Similarly, destructive interference can only happen without the presence of the suprathreshold spikes. (D) The suprathreshold spike was triggered in the temporal region of the slice and propagated to the septal region. (E) Similarly, the suprathreshold spikes can be triggered in the septal area and traveled to the temporal area. (F) When the laser was applied on both sides of the slice and generated suprathreshold spikes simultaneously, only one spike was recorded through the slice instead of two spikes. (G) The normalized amplitudes of the spikes triggered by the laser from the left side, right side, or both sides were not significantly different. (H) The pulse widths of the spikes triggered by the laser from the left side, right side, or both sides were similar. (I) By analyzing the amplitudes from the signals in each condition, the amplitudes of these suprathreshold spikes were similar. However, the estimated amplitude was higher than the real amplitude of the spike when assuming the spikes can interfere with each other (**: p < 0.05, n = 3 slices).